Sdílet
We propose our expertise in tissue culture for the transformation of Brassica napus (canola, rapeseed) or B. oleracea with your plasmids of interest in order to generate transgenic plants. At the moment, the methodology is proposed in specific cultivars for which we have optimized our protocols. Contact us to discuss the possibility of testing and optimizing our method on your cultivars of choice.
This technology can be used for:
- Generating transgenic plants
- Agrobiotech research
Methods used
Explants are collected from cotyledon petioles of 5 day-old seedlings. The wounded part of the petiole is co-cultivated with a solution of Agrobacterium tumefaciens containing the transgene plasmid to be introduced into the plant. Wounded cells are growing into callus. The callus is then selected for the presence of the transgene. From the transformed callus, plants are clonally regenerated by cultures in successive growth media. When a plantlet with root and shoot is developed, it is transferred to the green house for seed production. The whole procedure takes 8 to 10 months.
Equipment
- Growth chambers
- Greenhouse
- Sterile flow bench
